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cell insight cx7 high-content screening (hcs) platform  (Thermo Fisher)


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    Thermo Fisher cell insight cx7 high-content screening (hcs) platform
    Cell Insight Cx7 High Content Screening (Hcs) Platform, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+insight+cx7+high-content+screening+(hcs)+platform/bio_rxiv__2024__09__12__612614-248-28-35
    Average 90 stars, based on 1 article reviews
    cell insight cx7 high-content screening (hcs) platform - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: SARS-CoV-2 protein ORF3a induces Atg8ylation of lysosomal membranes
    Article Snippet: Cells were then incubated in full media and incubated with Hoechst 33342 for ten minutes and then acquired for Keima fluorescence at 440nm and 560 nm using the Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo) .

    Article Title: p32/C1QBP regulates OMA1-dependent proteolytic processing of OPA1 to maintain mitochondrial connectivity related to mitochondrial dysfunction and apoptosis
    Article Snippet: Cultured spheroids were stained with 2 μM calcein-AM and 4 μM Ethidium homodimer-1 (EthD-1) incubated simultaneously at 37 °C for 1 h. Analysis of labeled cells was performed using Cell Insight CX7 LZR high-content screening (HCS) Platform (Thermo Scientific USA, MA, USA).

    Article Title: Mammalian ATG8 proteins maintain autophagosomal membrane integrity through ESCRTs.
    Article Snippet: The canonical autophagy pathway in mammalian cells sequesters diverse cytoplasmic cargo within the double membrane autophagosomes that eventually convert into degradative compartments via fusion with endolysosomal intermediates.. Here, we report that autophagosomal membranes show permeability in cells lacking principal ATG8 proteins (mATG8s) and are unable to mature into autolysosomes.. Using a combination of methods including a novel in vitro assay to measure membrane sealing, we uncovered a previously unappreciated function of mATG8s to maintain autophagosomal membranes in a sealed state.

    Article Title: Phosphorylation of Syntaxin 17 by TBK1 controls autophagy initiation
    Article Snippet: After autophagy induction cells were incubated with Hoechst 33342 for ten minutes and then images acquired for Keima fluorescence at 440 nm and 560 nm using the Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Fisher Scientific).

    Article Title: Toxoplasma -proximal and distal control by GBPs in human macrophages
    Article Snippet: Plates were imaged on a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Scientific) using 20× magnification.

    Article Title: Toxoplasma -proximal and distal control by GBPs in human macrophages
    Article Snippet: Plates were imaged on a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Scientific) using 20x magnification.

    Article Title: CD73, a Promising Therapeutic Target of Diclofenac, Promotes Metastasis of Pancreatic Cancer through a Nucleotidase Independent Mechanism
    Article Snippet: 4000 cells were seeded in a 24‐well plate for 24 h and then automatically tracked for 16 h by the Cell Insight CX7 High‐Content Screening (HCS) Platform (ThermoFisher, USA).

    Article Title: Phenotypic Consequences of SLC25A40-ABCB1 Fusions beyond Drug Resistance in High-Grade Serous Ovarian Cancer
    Article Snippet: For cell viability, the entire area of each well was imaged (9 fields) at 10× magnification, and for actin morphology, ~300 cells were imaged at 20× magnification using a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Fisher Scientific, Waltham, MA, USA).

    Fluorescence:

    Article Title: SARS-CoV-2 protein ORF3a induces Atg8ylation of lysosomal membranes
    Article Snippet: Cells were then incubated in full media and incubated with Hoechst 33342 for ten minutes and then acquired for Keima fluorescence at 440nm and 560 nm using the Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo) .

    Article Title: p32/C1QBP regulates OMA1-dependent proteolytic processing of OPA1 to maintain mitochondrial connectivity related to mitochondrial dysfunction and apoptosis
    Article Snippet: Cultured spheroids were stained with 2 μM calcein-AM and 4 μM Ethidium homodimer-1 (EthD-1) incubated simultaneously at 37 °C for 1 h. Analysis of labeled cells was performed using Cell Insight CX7 LZR high-content screening (HCS) Platform (Thermo Scientific USA, MA, USA).

    Article Title: Mammalian ATG8 proteins maintain autophagosomal membrane integrity through ESCRTs.
    Article Snippet: The canonical autophagy pathway in mammalian cells sequesters diverse cytoplasmic cargo within the double membrane autophagosomes that eventually convert into degradative compartments via fusion with endolysosomal intermediates.. Here, we report that autophagosomal membranes show permeability in cells lacking principal ATG8 proteins (mATG8s) and are unable to mature into autolysosomes.. Using a combination of methods including a novel in vitro assay to measure membrane sealing, we uncovered a previously unappreciated function of mATG8s to maintain autophagosomal membranes in a sealed state.

    Article Title: Phosphorylation of Syntaxin 17 by TBK1 controls autophagy initiation
    Article Snippet: After autophagy induction cells were incubated with Hoechst 33342 for ten minutes and then images acquired for Keima fluorescence at 440 nm and 560 nm using the Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Fisher Scientific).

    Article Title: Toxoplasma -proximal and distal control by GBPs in human macrophages
    Article Snippet: Plates were imaged on a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Scientific) using 20× magnification.

    Article Title: Toxoplasma -proximal and distal control by GBPs in human macrophages
    Article Snippet: Plates were imaged on a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Scientific) using 20x magnification.

    Article Title: CD73, a Promising Therapeutic Target of Diclofenac, Promotes Metastasis of Pancreatic Cancer through a Nucleotidase Independent Mechanism
    Article Snippet: 4000 cells were seeded in a 24‐well plate for 24 h and then automatically tracked for 16 h by the Cell Insight CX7 High‐Content Screening (HCS) Platform (ThermoFisher, USA).

    Article Title: Phenotypic Consequences of SLC25A40-ABCB1 Fusions beyond Drug Resistance in High-Grade Serous Ovarian Cancer
    Article Snippet: For cell viability, the entire area of each well was imaged (9 fields) at 10× magnification, and for actin morphology, ~300 cells were imaged at 20× magnification using a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Fisher Scientific, Waltham, MA, USA).

    High Content Screening:

    Article Title: SARS-CoV-2 protein ORF3a induces Atg8ylation of lysosomal membranes
    Article Snippet: Cells were then incubated in full media and incubated with Hoechst 33342 for ten minutes and then acquired for Keima fluorescence at 440nm and 560 nm using the Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo) .

    Article Title: p32/C1QBP regulates OMA1-dependent proteolytic processing of OPA1 to maintain mitochondrial connectivity related to mitochondrial dysfunction and apoptosis
    Article Snippet: Cultured spheroids were stained with 2 μM calcein-AM and 4 μM Ethidium homodimer-1 (EthD-1) incubated simultaneously at 37 °C for 1 h. Analysis of labeled cells was performed using Cell Insight CX7 LZR high-content screening (HCS) Platform (Thermo Scientific USA, MA, USA).

    Article Title: Mammalian ATG8 proteins maintain autophagosomal membrane integrity through ESCRTs.
    Article Snippet: The canonical autophagy pathway in mammalian cells sequesters diverse cytoplasmic cargo within the double membrane autophagosomes that eventually convert into degradative compartments via fusion with endolysosomal intermediates.. Here, we report that autophagosomal membranes show permeability in cells lacking principal ATG8 proteins (mATG8s) and are unable to mature into autolysosomes.. Using a combination of methods including a novel in vitro assay to measure membrane sealing, we uncovered a previously unappreciated function of mATG8s to maintain autophagosomal membranes in a sealed state.

    Article Title: Phosphorylation of Syntaxin 17 by TBK1 controls autophagy initiation
    Article Snippet: After autophagy induction cells were incubated with Hoechst 33342 for ten minutes and then images acquired for Keima fluorescence at 440 nm and 560 nm using the Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Fisher Scientific).

    Article Title: Toxoplasma -proximal and distal control by GBPs in human macrophages
    Article Snippet: Plates were imaged on a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Scientific) using 20× magnification.

    Article Title: Toxoplasma -proximal and distal control by GBPs in human macrophages
    Article Snippet: Plates were imaged on a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Scientific) using 20x magnification.

    Article Title: CD73, a Promising Therapeutic Target of Diclofenac, Promotes Metastasis of Pancreatic Cancer through a Nucleotidase Independent Mechanism
    Article Snippet: 4000 cells were seeded in a 24‐well plate for 24 h and then automatically tracked for 16 h by the Cell Insight CX7 High‐Content Screening (HCS) Platform (ThermoFisher, USA).

    Article Title: Phenotypic Consequences of SLC25A40-ABCB1 Fusions beyond Drug Resistance in High-Grade Serous Ovarian Cancer
    Article Snippet: For cell viability, the entire area of each well was imaged (9 fields) at 10× magnification, and for actin morphology, ~300 cells were imaged at 20× magnification using a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Fisher Scientific, Waltham, MA, USA).

    Cell Culture:

    Article Title: SARS-CoV-2 protein ORF3a induces Atg8ylation of lysosomal membranes
    Article Snippet: Cells were then incubated in full media and incubated with Hoechst 33342 for ten minutes and then acquired for Keima fluorescence at 440nm and 560 nm using the Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo) .

    Article Title: p32/C1QBP regulates OMA1-dependent proteolytic processing of OPA1 to maintain mitochondrial connectivity related to mitochondrial dysfunction and apoptosis
    Article Snippet: Cultured spheroids were stained with 2 μM calcein-AM and 4 μM Ethidium homodimer-1 (EthD-1) incubated simultaneously at 37 °C for 1 h. Analysis of labeled cells was performed using Cell Insight CX7 LZR high-content screening (HCS) Platform (Thermo Scientific USA, MA, USA).

    Article Title: Mammalian ATG8 proteins maintain autophagosomal membrane integrity through ESCRTs.
    Article Snippet: The canonical autophagy pathway in mammalian cells sequesters diverse cytoplasmic cargo within the double membrane autophagosomes that eventually convert into degradative compartments via fusion with endolysosomal intermediates.. Here, we report that autophagosomal membranes show permeability in cells lacking principal ATG8 proteins (mATG8s) and are unable to mature into autolysosomes.. Using a combination of methods including a novel in vitro assay to measure membrane sealing, we uncovered a previously unappreciated function of mATG8s to maintain autophagosomal membranes in a sealed state.

    Article Title: Phosphorylation of Syntaxin 17 by TBK1 controls autophagy initiation
    Article Snippet: After autophagy induction cells were incubated with Hoechst 33342 for ten minutes and then images acquired for Keima fluorescence at 440 nm and 560 nm using the Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Fisher Scientific).

    Article Title: Toxoplasma -proximal and distal control by GBPs in human macrophages
    Article Snippet: Plates were imaged on a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Scientific) using 20× magnification.

    Article Title: Toxoplasma -proximal and distal control by GBPs in human macrophages
    Article Snippet: Plates were imaged on a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Scientific) using 20x magnification.

    Article Title: CD73, a Promising Therapeutic Target of Diclofenac, Promotes Metastasis of Pancreatic Cancer through a Nucleotidase Independent Mechanism
    Article Snippet: 4000 cells were seeded in a 24‐well plate for 24 h and then automatically tracked for 16 h by the Cell Insight CX7 High‐Content Screening (HCS) Platform (ThermoFisher, USA).

    Article Title: Phenotypic Consequences of SLC25A40-ABCB1 Fusions beyond Drug Resistance in High-Grade Serous Ovarian Cancer
    Article Snippet: For cell viability, the entire area of each well was imaged (9 fields) at 10× magnification, and for actin morphology, ~300 cells were imaged at 20× magnification using a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Fisher Scientific, Waltham, MA, USA).

    Staining:

    Article Title: SARS-CoV-2 protein ORF3a induces Atg8ylation of lysosomal membranes
    Article Snippet: Cells were then incubated in full media and incubated with Hoechst 33342 for ten minutes and then acquired for Keima fluorescence at 440nm and 560 nm using the Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo) .

    Article Title: p32/C1QBP regulates OMA1-dependent proteolytic processing of OPA1 to maintain mitochondrial connectivity related to mitochondrial dysfunction and apoptosis
    Article Snippet: Cultured spheroids were stained with 2 μM calcein-AM and 4 μM Ethidium homodimer-1 (EthD-1) incubated simultaneously at 37 °C for 1 h. Analysis of labeled cells was performed using Cell Insight CX7 LZR high-content screening (HCS) Platform (Thermo Scientific USA, MA, USA).

    Article Title: Mammalian ATG8 proteins maintain autophagosomal membrane integrity through ESCRTs.
    Article Snippet: The canonical autophagy pathway in mammalian cells sequesters diverse cytoplasmic cargo within the double membrane autophagosomes that eventually convert into degradative compartments via fusion with endolysosomal intermediates.. Here, we report that autophagosomal membranes show permeability in cells lacking principal ATG8 proteins (mATG8s) and are unable to mature into autolysosomes.. Using a combination of methods including a novel in vitro assay to measure membrane sealing, we uncovered a previously unappreciated function of mATG8s to maintain autophagosomal membranes in a sealed state.

    Article Title: Phosphorylation of Syntaxin 17 by TBK1 controls autophagy initiation
    Article Snippet: After autophagy induction cells were incubated with Hoechst 33342 for ten minutes and then images acquired for Keima fluorescence at 440 nm and 560 nm using the Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Fisher Scientific).

    Article Title: Toxoplasma -proximal and distal control by GBPs in human macrophages
    Article Snippet: Plates were imaged on a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Scientific) using 20× magnification.

    Article Title: Toxoplasma -proximal and distal control by GBPs in human macrophages
    Article Snippet: Plates were imaged on a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Scientific) using 20x magnification.

    Article Title: CD73, a Promising Therapeutic Target of Diclofenac, Promotes Metastasis of Pancreatic Cancer through a Nucleotidase Independent Mechanism
    Article Snippet: 4000 cells were seeded in a 24‐well plate for 24 h and then automatically tracked for 16 h by the Cell Insight CX7 High‐Content Screening (HCS) Platform (ThermoFisher, USA).

    Article Title: Phenotypic Consequences of SLC25A40-ABCB1 Fusions beyond Drug Resistance in High-Grade Serous Ovarian Cancer
    Article Snippet: For cell viability, the entire area of each well was imaged (9 fields) at 10× magnification, and for actin morphology, ~300 cells were imaged at 20× magnification using a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Fisher Scientific, Waltham, MA, USA).

    Ethidium Homodimer Assay:

    Article Title: SARS-CoV-2 protein ORF3a induces Atg8ylation of lysosomal membranes
    Article Snippet: Cells were then incubated in full media and incubated with Hoechst 33342 for ten minutes and then acquired for Keima fluorescence at 440nm and 560 nm using the Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo) .

    Article Title: p32/C1QBP regulates OMA1-dependent proteolytic processing of OPA1 to maintain mitochondrial connectivity related to mitochondrial dysfunction and apoptosis
    Article Snippet: Cultured spheroids were stained with 2 μM calcein-AM and 4 μM Ethidium homodimer-1 (EthD-1) incubated simultaneously at 37 °C for 1 h. Analysis of labeled cells was performed using Cell Insight CX7 LZR high-content screening (HCS) Platform (Thermo Scientific USA, MA, USA).

    Article Title: Mammalian ATG8 proteins maintain autophagosomal membrane integrity through ESCRTs.
    Article Snippet: The canonical autophagy pathway in mammalian cells sequesters diverse cytoplasmic cargo within the double membrane autophagosomes that eventually convert into degradative compartments via fusion with endolysosomal intermediates.. Here, we report that autophagosomal membranes show permeability in cells lacking principal ATG8 proteins (mATG8s) and are unable to mature into autolysosomes.. Using a combination of methods including a novel in vitro assay to measure membrane sealing, we uncovered a previously unappreciated function of mATG8s to maintain autophagosomal membranes in a sealed state.

    Article Title: Phosphorylation of Syntaxin 17 by TBK1 controls autophagy initiation
    Article Snippet: After autophagy induction cells were incubated with Hoechst 33342 for ten minutes and then images acquired for Keima fluorescence at 440 nm and 560 nm using the Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Fisher Scientific).

    Article Title: Toxoplasma -proximal and distal control by GBPs in human macrophages
    Article Snippet: Plates were imaged on a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Scientific) using 20× magnification.

    Article Title: Toxoplasma -proximal and distal control by GBPs in human macrophages
    Article Snippet: Plates were imaged on a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Scientific) using 20x magnification.

    Article Title: CD73, a Promising Therapeutic Target of Diclofenac, Promotes Metastasis of Pancreatic Cancer through a Nucleotidase Independent Mechanism
    Article Snippet: 4000 cells were seeded in a 24‐well plate for 24 h and then automatically tracked for 16 h by the Cell Insight CX7 High‐Content Screening (HCS) Platform (ThermoFisher, USA).

    Article Title: Phenotypic Consequences of SLC25A40-ABCB1 Fusions beyond Drug Resistance in High-Grade Serous Ovarian Cancer
    Article Snippet: For cell viability, the entire area of each well was imaged (9 fields) at 10× magnification, and for actin morphology, ~300 cells were imaged at 20× magnification using a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Fisher Scientific, Waltham, MA, USA).

    Labeling:

    Article Title: SARS-CoV-2 protein ORF3a induces Atg8ylation of lysosomal membranes
    Article Snippet: Cells were then incubated in full media and incubated with Hoechst 33342 for ten minutes and then acquired for Keima fluorescence at 440nm and 560 nm using the Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo) .

    Article Title: p32/C1QBP regulates OMA1-dependent proteolytic processing of OPA1 to maintain mitochondrial connectivity related to mitochondrial dysfunction and apoptosis
    Article Snippet: Cultured spheroids were stained with 2 μM calcein-AM and 4 μM Ethidium homodimer-1 (EthD-1) incubated simultaneously at 37 °C for 1 h. Analysis of labeled cells was performed using Cell Insight CX7 LZR high-content screening (HCS) Platform (Thermo Scientific USA, MA, USA).

    Article Title: Mammalian ATG8 proteins maintain autophagosomal membrane integrity through ESCRTs.
    Article Snippet: The canonical autophagy pathway in mammalian cells sequesters diverse cytoplasmic cargo within the double membrane autophagosomes that eventually convert into degradative compartments via fusion with endolysosomal intermediates.. Here, we report that autophagosomal membranes show permeability in cells lacking principal ATG8 proteins (mATG8s) and are unable to mature into autolysosomes.. Using a combination of methods including a novel in vitro assay to measure membrane sealing, we uncovered a previously unappreciated function of mATG8s to maintain autophagosomal membranes in a sealed state.

    Article Title: Phosphorylation of Syntaxin 17 by TBK1 controls autophagy initiation
    Article Snippet: After autophagy induction cells were incubated with Hoechst 33342 for ten minutes and then images acquired for Keima fluorescence at 440 nm and 560 nm using the Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Fisher Scientific).

    Article Title: Toxoplasma -proximal and distal control by GBPs in human macrophages
    Article Snippet: Plates were imaged on a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Scientific) using 20× magnification.

    Article Title: Toxoplasma -proximal and distal control by GBPs in human macrophages
    Article Snippet: Plates were imaged on a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Scientific) using 20x magnification.

    Article Title: CD73, a Promising Therapeutic Target of Diclofenac, Promotes Metastasis of Pancreatic Cancer through a Nucleotidase Independent Mechanism
    Article Snippet: 4000 cells were seeded in a 24‐well plate for 24 h and then automatically tracked for 16 h by the Cell Insight CX7 High‐Content Screening (HCS) Platform (ThermoFisher, USA).

    Article Title: Phenotypic Consequences of SLC25A40-ABCB1 Fusions beyond Drug Resistance in High-Grade Serous Ovarian Cancer
    Article Snippet: For cell viability, the entire area of each well was imaged (9 fields) at 10× magnification, and for actin morphology, ~300 cells were imaged at 20× magnification using a Cell Insight CX7 High-Content Screening (HCS) Platform (Thermo Fisher Scientific, Waltham, MA, USA).



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